Structural organization of polygalacturonase-encoding genes from Penicillium griseoroseum
نویسندگان
چکیده
منابع مشابه
Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene
Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putativ...
متن کاملMorphological and Molecular Differentiation of the Pectinase Producing Fungi Penicillium Expansum and Penicillium Griseoroseum
Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers ...
متن کاملFunctional Screening of Phosphatase-Encoding Genes from Bacterial Sources
Phosphatase (APase) enzymes including phytases have broad applications in diagnostic kits, poultryfeeds, biofertilizers and plant nutrition. Because of high levels of sequence diversity among phosphatases,an efficient functional screening method is a crucial requirement for the isolation of the encodinggenes. This study reports a functional cloning screening method for the iso...
متن کاملCloning and targeted disruption of two polygalacturonase genes in Penicillium olsonii.
The filamentous fungus Penicillium olsonii secretes several polygalacturonases (PGs) with molecular masses of about 47 kDa. These enzymes consist of several basic and acidic isoforms, with dominant activities at pI 4.5 and pI 7.9. Two polygalacturonase genes, pg1 and pg2, have been cloned. The corresponding enzymes, PG1 and PG2, consist of 370 and 380 amino acids, respectively, and show signifi...
متن کاملThe pectin-degrading system of Penicillium cupsulatum: characterization of an extracellular polygalacturonase from solid-state cultures
60 min to 474.1 f 61.5 fmol of Caz+/mg cartilage, whereas boiling the cartilage for 15 min before incubation failed to alter the amount of CaZ+ taken up. Sodium orthovanadate (0.05 mM), a potent inhibitor of CaZ+-stimulated ATPase (ONeal et al., 1979), failed to alter the uptake of Ca2+ into cartilage. Similarly, the uptake of Ca2+ into cartilage was not modified by prior incubation with diflun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetics and Molecular Biology
سال: 2002
ISSN: 1415-4757
DOI: 10.1590/s1415-47572002000400020